Alkaptonuria is a rare genetic disorder that affects the production of an enzyme called homogentisic acid oxidase. This enzyme is responsible for breaking down the amino acid tyrosine. When it is not functioning properly, the body is unable to break down tyrosine, resulting in a buildup of the acid in the body. As a result, people with alkaptonuria experience a variety of symptoms, including dark urine, joint pain, and arthritis.
The alkali test is a diagnostic tool used to confirm the diagnosis of alkaptonuria. It involves adding a base, such as sodium hydroxide, to a sample of urine. If the test is positive, a brownish-black discoloration will appear in the sample. This indicates the presence of homogentisic acid, which is a byproduct of tyrosine metabolism and is only present in people with alkaptonuria.
The positive result for alkaptonuria in alkali test is a brownish-black discoloration of the sample. This discoloration is caused by the presence of homogentisic acid, which is a byproduct of tyrosine metabolism. The alkali test is an easy and reliable way to diagnose alkaptonuria, as it is a definitive indicator of the disorder.