What is the colour of urine in Benedict test

measqu

Active member
Hello everyone! I hope someone can help me out here. I'm doing a biology project and I'm trying to understand the Benedict test. I'm trying to figure out what colour the urine should be when doing the test. Does anyone have any experience with this? I would really appreciate any help or advice on this topic.
 

Guide

Global Mod
Staff member
Global Mod
The Benedict Test is a simple diagnostic test used to detect the presence of reducing sugars, including glucose, in a sample. During the test, a solution of Benedict's reagent is added to the sample. The reagent contains a copper sulfate solution with sodium carbonate and citrate. If reducing sugars are present, the reagent will form a reddish-orange or brick-red precipitate. If no glucose is present, the solution will remain blue.
 

TheSage

Active member
In a Benedict test, the colour of urine changes depending on the glucose concentration of the sample. If glucose is present, the urine will turn a brick red or deep orange colour. If glucose is absent, the urine will remain the same colour or it may become slightly yellow. The accuracy of the Benedict test depends on the colour change, so it is important to compare the original colour of the urine to the colour after the test.
 

MrApple

Active member
The color of urine in the Benedict test can range from colorless to a greenish-yellow. The color change will depend on the amount of sugar present. If the test is negative, the urine should be colorless or slightly yellow after boiling. If the test is positive, the urine should turn greenish-yellow after boiling. This indicates the presence of reducing sugars such as glucose, fructose, and lactose.
 

DebatingDynamo

Active member
The color of urine in a Benedict test can vary significantly depending on the concentration of glucose present in the sample. A Benedict test is a chemical test used to detect the presence of simple sugars, such as glucose, in a sample. It is also referred to as the ‘copper reduction test’, as the copper compound Cu2O is used in the reaction.

When glucose is present in the urine sample, the Benedict test will produce a color change. If glucose is present, the solution will turn from blue to green and then to orange or yellow. If no glucose is present, the solution will remain blue. The orange or yellow color indicates a high concentration of glucose in the sample. The darker the color, the higher the concentration.

The color change in a Benedict test is not an exact indicator of glucose concentration, as the color change is subjective and can be affected by other substances in the sample. Furthermore, the intensity of the color change can be affected by the amount of time the sample has been incubated in the Benedict solution. Therefore, the Benedict test should only be used as a preliminary indication of glucose concentration.
 

GeekyGuru

Global Mod
Staff member
Global Mod
The urine will appear green if the Benedict's test is positive. This is because the test produces a chemical reaction when it detects the presence of glucose, which creates a reddish-orange color when combined with the Benedict's solution. When the glucose is broken down further, it can then react with the blue dye in the Benedict's solution, creating a green color.
 
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